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At ImQuest BioSciences, we can support your early- to late-stage preclinical protein drug development. From protein expression optimization to purification protocol design through to analytical and bioanalytical characterizations, ImQuest can guide your protein to an IND.
ImQuest has expertise in optimizing protein expression systems to increase expression, yield, and purity, while decreasing heterogeneity and unwanted posttranslational modifications. ImQuest is also proficient in protein purification as well as process development and optimization. Our team can analyze the identity, purity, stability (preformulation studies), and activity of your protein or peptide |
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Protein Production:
We can perform, develop, and/or optimize the cloning, expression, and purification of your protein or peptide drug, including process development and optimization. We can aid in the design of your protein or peptide in order to increase expression levels, increase potency, increase stability, and/or decrease heterogeneity.
Protein Analytical Services:
We can develop the necessary methods to analyze your protein or peptide. We can analyze the purity and identity of your protein (1D and 2D SDS-PAGE, IEF, chromatofocusing, FPLC/HPLC, Western blotting, ELISA); perform preformulation and protein stability studies (pH and ionic strength, buffer and excipient compatibility, forced degradation and stability); perform activity analyses using bioassays or enzymatic assays (cell- or non-cell based); complete other biochemical and biophysical analyses.
Protein Bioanalytical Services:
We can analyze your protein using immunoassays, detection of immunogenicity and/or neutralizing antibodies, or use other assays as customized to your development needs.
Protein Therapeutic Support:
ImQuest can support the development of your protein drug from discovery to the late stage preclinical phase, aiding in project progression and IND submission using ICH Guidelines and FDA Points to Consider. |
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Enzymology
ImQuest’s core enzymology capabilities can provide quantitative determination of kinetic parameters for substrates and inhibitors. Our standard and customized enzymatic assays can be performed with wild-type or resistant enzymes. ImQuest can tailor its services to meet your needs for the development or optimization of new assays. ImQuest also offers a wide array of bioanalytical services, such as immunogenicity and neutralization studies, and therapeutic protein development support.
Standard Antiviral Enzyme Assays:
We will perform standard antiviral enzyme assays to analyze the potency of your drug against viral enzymes, such as reverse transcriptase, integrase, protease, RNase H, and polymerases. We can analyze the potency of your drug against wild-type or drug-resistant viral enzymes in vitro with purified components or in cell-based antiviral assays.
Host Cell Polymerase Assays:
We will perform standard polymerase assays to aid in the prediction of cellular toxicity.
Assay Development and Optimization:
We can develop or optimize your enzyme assay, and customize our analyses to meet your project needs.
Kinetic Parameters and Inhibition Studies:
We can analyze your enzyme inhibitor in simple screens for potency, or we can kinetically determine the inhibition constant (Ki) and type of inhibition in vitro. We can also analyze the effect your inhibitor has on the kinetic parameters of the enzyme (KM, Vmax, kcat).
Drug Development Support:
ImQuest can support the development of your drug substance or drug product from discovery to late stage preclinical phases, aiding in project progression and IND submission using ICH Guidelines and FDA Points to Consider. |
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